Herpes viral culture of lesion

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Viral lesion culture

Herpes viral culture of a lesion is a test to confirm the presence of herpes simplex virus in a skin sore.

Culture - herpes simplex virus; Herpes simplex virus culture

A specimen from a skin lesion (often a genital sore) is collected during the acute phase (worst part) of an outbreak and placed in a special substance called medium.

The specimen may be placed in cell cultures, so they can replicate to help identification, or it may be identified by monoclonal antibodies that detect early antigens of herpes simplex virus.

Serotyping of the virus (to determine whether it's type 1 or 2) may also be done. Results are available within 16 hours to 7 days, depending on the culture method used.

The specimen must be collected during an acute infection.

The specimen is collected by scraping the suspected skin lesion or aspirating fluid from the lesion. This can cause a scraping or sticking sensation. Sometimes a specimen from the throat or eyes is obtained, usually by rubbing a sterile swab against the tissues.

The test is performed to confirm herpes simplex infection. The diagnosis is often made by clinical examination rather than diagnostic testing.

Review Date: 02/10/2006
Reviewed By: Kenneth Wener, M.D., Division of Infectious Diseases, Beth Israel Deaconess Medical Center, Boston, MA. Review provided by VeriMed Healthcare Network.